› 1zz8

X-ray diffraction
2.3Å resolution

Crystal Structure of FeII HppE in Complex with Substrate Form 2

Released:
DOI: 10.2210/pdb1zz8/pdb
PDB entry 1zz8 coloured by chain, front view.
PDB entry 1zz8 coloured by chain, side view.
PDB entry 1zz8 coloured by chain, top view.
Source organism: Streptomyces wedmorensis
Primary publication:
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme.
Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL
Nature 437 838-44 (2005)
PMID: 16015285

Function and Biology Details

Reaction catalysed: 
(S)-2-hydroxypropylphosphonate + H(2)O(2) = (1R,2S)-1,2-epoxypropylphosphonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-hydroxypropylphosphonic acid epoxidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 198 amino acids
Theoretical weight: 21.36 KDa
Source organism: Streptomyces wedmorensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56185 (Residues: 1-198; Coverage: 100%)
Gene names: fom4, hppE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 3 x FE2
Ligand S0H 3 x S0H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P42212
Unit cell:
a: 111.649Å b: 111.649Å c: 152.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
14 more

4 mentions without citation

Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis.
Yun et al. (2011)
3 more