› 242d

X-ray diffraction
1.65Å resolution

MAD PHASING STRATEGIES EXPLORED WITH A BROMINATED OLIGONUCLEOTIDE CRYSTAL AT 1.65 A RESOLUTION.

Released:
DOI: 10.2210/pdb242d/pdb
PDB entry 242d coloured by chain, front view.
PDB entry 242d coloured by chain, side view.
PDB entry 242d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
MAD Phasing Strategies Explored with a Brominated Oligonucleotide Crystal at 1.65A Resolution.
Peterson MR, Harrop SJ, McSweeney SM, Leonard GA, Thompson AW, Hunter WN, Helliwell JR
J Synchrotron Radiat 3 24-34 (1996)
PMID: 16702655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115007 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*GP*(CBR)P*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 6 nucleotides
Theoretical weight: 1.89 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CBR 2 x CBR

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P212121
Unit cell:
a: 17.97Å b: 30.98Å c: 44.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 not available not available
Expression system: Not provided

Quick links

Citations

1 review citation

Trends and challenges in experimental macromolecular crystallography.
Chayen et al. (1996)
2 other articles

1 mention without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)