› 274d

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF A COVALENT DNA-DRUG ADDUCT: ANTHRAMYCIN BOUND TO C-C-A-A-C-G-T-T-G-G, AND A MOLECULAR EXPLANATION OF SPECIFICITY

Released:
DOI: 10.2210/pdb274d/pdb
PDB entry 274d coloured by chain, front view.
PDB entry 274d coloured by chain, side view.
PDB entry 274d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of a covalent DNA-drug adduct: anthramycin bound to C-C-A-A-C-G-T-T-G-G and a molecular explanation of specificity.
Kopka ML, Goodsell DS, Baikalov I, Grzeskowiak K, Cascio D, Dickerson RE
Biochemistry 33 13593-610 (1994)
PMID: 7947769

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115032 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*CP*AP*AP*CP*GP*TP*TP*(DRUG)GP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ANT 1 x ANT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 23.95Å b: 23.95Å c: 88.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 not available not available
Expression system: Not provided

Quick links

Citations

11 review citations

Synthetic DNA minor groove-binding drugs.
Reddy et al. (1999)
10 more

5 mentions without citation

From Anthramycin to Pyrrolobenzodiazepine (PBD)-Containing Antibody-Drug Conjugates (ADCs).
Mantaj et al. (2017)
4 more