› 2boj

X-ray diffraction
1.8Å resolution

crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with methyl-B-D-Arabinopyranoside

Released:
DOI: 10.2210/pdb2boj/pdb
PDB entry 2boj coloured by chain, front view.
PDB entry 2boj coloured by chain, side view.
PDB entry 2boj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures.
Sabin C, Mitchell EP, Pokorná M, Gautier C, Utille JP, Wimmerová M, Imberty A
FEBS Lett 580 982-7 (2006)
PMID: 16438968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.73 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 2-115; Coverage: 99%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

3 bound ligands:
Ligand ARW 4 x ARW
Ligand CA 8 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 50.636Å b: 80.168Å c: 52.517Å
α: 90° β: 109.92° γ: 90°
R-values:
R R work R free
0.137 0.136 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

From carbohydrate leads to glycomimetic drugs.
Ernst et al. (2009)
8 more

1 mention without citation

Predicting binding sites from unbound versus bound protein structures.
Clark et al. (2020)