› 2bs7

X-ray diffraction
2.1Å resolution

Crystal structure of F17b-G in complex with chitobiose

Released:
DOI: 10.2210/pdb2bs7/pdb
PDB entry 2bs7 coloured by chain, front view.
PDB entry 2bs7 coloured by chain, side view.
PDB entry 2bs7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Impact of natural variation in bacterial F17G adhesins on crystallization behaviour.
Buts L, Wellens A, Van Molle I, Wyns L, Loris R, Lahmann M, Oscarson S, De Greve H, Bouckaert J
Acta Crystallogr D Biol Crystallogr 61 1149-59 (2005)
PMID: 16041081

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
F17b-G fimbrial adhesin Chain: 1
Molecule details ›
Chain: 1
Length: 176 amino acids
Theoretical weight: 18.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47200 (Residues: 23-198; Coverage: 55%)
Gene name: f17bG
Sequence domains: Fimbrial adhesin F17-AG, lectin domain
Structure domains: Bacterial adhesins - F17c-type

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P62
Unit cell:
a: 86.698Å b: 86.698Å c: 57.043Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

3D deep convolutional neural networks for amino acid environment similarity analysis.
Torng et al. (2017)
1 more

1 mention without citation

Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.
Lonardi et al. (2013)