› 2g7c

X-ray diffraction
2Å resolution

Clostridium difficile Toxin A Fragment Bound to aGal(1,3)bGal(1,4)bGlcNAc

Released:
DOI: 10.2210/pdb2g7c/pdb
PDB entry 2g7c coloured by chain, front view.
PDB entry 2g7c coloured by chain, side view.
PDB entry 2g7c coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Carbohydrate recognition by Clostridium difficile toxin A.
Greco A, Ho JG, Lin SJ, Palcic MM, Rupnik M, Ng KK
Nat Struct Mol Biol 13 460-1 (2006)
PMID: 16622409

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Toxin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.64 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P16154 (Residues: 2456-2710; Coverage: 9%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Cholin Binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, GLA
Carbohydrate polymer
1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 38.682Å b: 132.825Å c: 65.917Å
α: 90° β: 100.29° γ: 90°
R-values:
R R work R free
0.197 0.195 0.236
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Clostridium difficile infection.
Smits et al. (2016)
23 more

7 mentions without citation

The role of toxins in Clostridium difficile infection.
Chandrasekaran et al. (2017)
6 more