› 2vxj

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH AGAL13BGAL14GLC AT 1.9 A RESOLUTION

Released:
DOI: 10.2210/pdb2vxj/pdb
PDB entry 2vxj coloured by chain, front view.
PDB entry 2vxj coloured by chain, side view.
PDB entry 2vxj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural basis of the preferential binding for globo-series glycosphingolipids displayed by Pseudomonas aeruginosa lectin I.
Blanchard B, Nurisso A, Hollville E, Tétaud C, Wiels J, Pokorná M, Wimmerová M, Varrot A, Imberty A
J Mol Biol 383 837-53 (2008)
PMID: 18762193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
PA-I galactophilic lectin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, GLA, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, GLA
Carbohydrate polymer
3 bound ligands:
Ligand CA 24 x CA
Ligand EDO 20 x EDO
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 79.184Å b: 86.521Å c: 119.078Å
α: 93.93° β: 98.16° γ: 90.08°
R-values:
R R work R free
0.188 0.185 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Glycan microarrays for decoding the glycome.
Rillahan et al. (2011)
12 more

6 mentions without citation

Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience.
Pérez et al. (2017)
5 more