› 2a0u

X-ray diffraction
2.1Å resolution

Crystal structure of the eukaryotic initiation factor 2B from Leishmania major at 2.1 A resolution

Released:
DOI: 10.2210/pdb2a0u/pdb
PDB entry 2a0u coloured by chain, front view.
PDB entry 2a0u coloured by chain, side view.
PDB entry 2a0u coloured by chain, top view.
Source organism: Leishmania major
Entry authors: Bosch J, Hol WGJ, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylthioribose-1-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 41.82 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4Q0R9 (Residues: 1-375; Coverage: 100%)
Gene names: LmjF36.4930, LmjF_36_4930
Sequence domains: Initiation factor 2 subunit family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: F222
Unit cell:
a: 103.733Å b: 164.569Å c: 172.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.264
Expression system: Escherichia coli

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