› 2a3d

Solution NMR

SOLUTION STRUCTURE OF A DE NOVO DESIGNED SINGLE CHAIN THREE-HELIX BUNDLE (A3D)

Released:
DOI: 10.2210/pdb2a3d/pdb
PDB entry 2a3d coloured by chain, front view.
PDB entry 2a3d coloured by chain, side view.
PDB entry 2a3d coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Solution structure and dynamics of a de novo designed three-helix bundle protein.
Walsh ST, Cheng H, Bryson JW, Roder H, DeGrado WF
Proc Natl Acad Sci U S A 96 5486-91 (1999)
PMID: 10318910

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PROTEIN (DE NOVO THREE-HELIX BUNDLE) Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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Citations

14 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
13 more

60 mentions without citation

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
59 more