› 2a46

X-ray diffraction
1.65Å resolution

Crystal structures of amFP486, a cyan fluorescent protein from Anemonia majano, and variants

Released:
DOI: 10.2210/pdb2a46/pdb
PDB entry 2a46 coloured by chain, front view.
PDB entry 2a46 coloured by chain, side view.
PDB entry 2a46 coloured by chain, top view.
Source organism: Anemonia manjano
Primary publication:
Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano.
Henderson JN, Remington SJ
Proc Natl Acad Sci U S A 102 12712-7 (2005)
PMID: 16120682

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein amFP486 Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 26.6 KDa
Source organism: Anemonia manjano
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y6 (Residues: 2-229; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand BME 1 x BME
1 modified residue:
Ligand CR7 1 x CR7

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: I4122
Unit cell:
a: 112.541Å b: 112.541Å c: 81.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.208
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
13 more

6 mentions without citation

Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
Ai et al. (2006)
5 more