› 2a5l

X-ray diffraction
1.7Å resolution

The crystal structure of the Trp repressor binding protein WrbA from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2a5l/pdb
PDB entry 2a5l coloured by chain, front view.
PDB entry 2a5l coloured by chain, side view.
PDB entry 2a5l coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Lunin VV, Evdokimova E, Kudritska M, Osipiuk J, Joachimiak A, Edwards AM, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-191399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H dehydrogenase (quinone) Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 21.16 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I509 (Residues: 1-198; Coverage: 100%)
Gene name: PA0949
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 62.927Å b: 62.927Å c: 190.022Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.196
Expression system: Escherichia coli BL21(DE3)

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