› 2a5y

X-ray diffraction
2.6Å resolution

Structure of a CED-4/CED-9 complex

Released:
DOI: 10.2210/pdb2a5y/pdb
PDB entry 2a5y coloured by chain, front view.
PDB entry 2a5y coloured by chain, side view.
PDB entry 2a5y coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structure of the CED-4-CED-9 complex provides insights into programmed cell death in Caenorhabditis elegans.
Yan N, Chai J, Lee ES, Gu L, Liu Q, He J, Wu JW, Kokel D, Li H, Hao Q, Xue D, Shi Y
Nature 437 831-7 (2005)
PMID: 16208361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151740 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator ced-9 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.72 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41958 (Residues: 48-251; Coverage: 73%)
Gene names: T07C4.8, ced-9
Sequence domains:
Structure domains: Blc2-like
Cell death protein 4 Chains: B, C
Molecule details ›
Chains: B, C
Length: 549 amino acids
Theoretical weight: 62.95 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P30429 (Residues: 1-571; Coverage: 96%)
Gene names: C35D10.9, ced-4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 128.898Å b: 128.898Å c: 209.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

44 review citations

The role of mitochondria in apoptosis*.
Wang et al. (2009)
43 more

20 mentions without citation

Regulation of the Apaf-1-caspase-9 apoptosome.
Bratton et al. (2010)
19 more