› 2a63

Solution NMR

Solution structure of a stably monomeric mutant of lambda Cro produced by substitutions in the ball-and-socket interface

Released:
DOI: 10.2210/pdb2a63/pdb
PDB entry 2a63 coloured by chain, ensemble of 20 models, front view.
PDB entry 2a63 coloured by chain, ensemble of 20 models, side view.
PDB entry 2a63 coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia virus Lambda
Primary publication:
A trade between similar but nonequivalent intrasubunit and intersubunit contacts in Cro dimer evolution.
Newlove T, Atkinson KR, Van Dorn LO, Cordes MH
Biochemistry 45 6379-91 (2006)
PMID: 16700549

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.42 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03040 (Residues: 1-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Transitive homology-guided structural studies lead to discovery of Cro proteins with 40% sequence identity but different folds.
Roessler et al. (2008)
6 more

2 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
1 more