› 2a6o

X-ray diffraction
2.6Å resolution

Crystal Structure of the ISHp608 Transposase in Complex with Stem-loop DNA

Released:
DOI: 10.2210/pdb2a6o/pdb
PDB entry 2a6o coloured by chain, front view.
PDB entry 2a6o coloured by chain, side view.
PDB entry 2a6o coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Active site sharing and subterminal hairpin recognition in a new class of DNA transposases.
Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F
Mol Cell 20 143-54 (2005)
PMID: 16209952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115063 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transposase IS200-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 18.11 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q933Z0 (Residues: 1-155; Coverage: 100%)
Gene names: B0X28_06285, B0X28_06415, B0X28_06965, B0X28_07035, B0X28_07355, B0X28_07380, B0X28_07400, B0X28_07410, B0X28_08670, B0X29_00655, B0X39_04170, BB413_07925, BB437_07715, DD756_06260, DDP35_01150, DDP42_08030, EC527_06560, EC565_08270, EPC71_00690, EPC72_02395, tnpA
Sequence domains: Transposase IS200 like
Structure domains: Transposase IS200-like
5'-D(*CP*CP*CP*CP*TP*AP*GP*CP*TP*TP*TP*AP*GP*CP*TP*AP*TP*GP*GP*GP*GP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.74 KDa
Source organism: Helicobacter pylori
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 44.333Å b: 71.132Å c: 76.746Å
α: 90° β: 94.44° γ: 90°
R-values:
R R work R free
0.209 0.209 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

ISCR elements: novel gene-capturing systems of the 21st century?
Toleman et al. (2006)
10 more

5 mentions without citation

A historical account of Hoogsteen base-pairs in duplex DNA.
Nikolova et al. (2013)
4 more