› 2a7s

X-ray diffraction
2.9Å resolution

Crystal Structure of the Acyl-CoA Carboxylase, AccD5, from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2a7s/pdb
PDB entry 2a7s coloured by chain, front view.
PDB entry 2a7s coloured by chain, side view.
PDB entry 2a7s coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure-based inhibitor design of AccD5, an essential acyl-CoA carboxylase carboxyltransferase domain of Mycobacterium tuberculosis.
Lin TW, Melgar MM, Kurth D, Swamidass SJ, Purdon J, Tseng T, Gago G, Baldi P, Gramajo H, Tsai SC
Proc Natl Acad Sci U S A 103 3072-7 (2006)
PMID: 16492739

Function and Biology Details

Reaction catalysed: 
[Biotin carboxyl-carrier protein]-N(6)-carboxybiotinyl-L-lysine + acetyl-CoA = [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine + malonyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase beta5 subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 548 amino acids
Theoretical weight: 59.42 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WQH7 (Residues: 1-548; Coverage: 100%)
Gene names: MTCY71.20, Rv3280, accD5, pccB
Sequence domains: Carboxyl transferase domain
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 175.252Å b: 175.252Å c: 342.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Mycolic acids: structures, biosynthesis, and beyond.
Marrakchi et al. (2014)
17 more

5 mentions without citation

Structure-based inhibitor design of AccD5, an essential acyl-CoA carboxylase carboxyltransferase domain of Mycobacterium tuberculosis.
Lin et al. (2006)
4 more