› 2a8k

X-ray diffraction
1.5Å resolution

Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease

Released:
DOI: 10.2210/pdb2a8k/pdb
PDB entry 2a8k coloured by chain, front view.
PDB entry 2a8k coloured by chain, side view.
PDB entry 2a8k coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and mutational studies of the catalytic domain of colicin E5: a tRNA-specific ribonuclease.
Lin YL, Elias Y, Huang RH
Biochemistry 44 10494-500 (2005)
PMID: 16060658

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 108 amino acids
Theoretical weight: 12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P18000 (Residues: 74-180; Coverage: 59%)
Gene name: col
Sequence domains: Colicin E5 ribonuclease domain
Structure domains: YaeB-like fold

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P31
Unit cell:
a: 65.22Å b: 65.22Å c: 100.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Colicin biology.
Cascales et al. (2007)
2 more

1 mention without citation

Structural basis for sequence-dependent recognition of colicin E5 tRNase by mimicking the mRNA-tRNA interaction.
Yajima et al. (2006)