› 2a92

X-ray diffraction
2.04Å resolution

Crystal structure of lactate dehydrogenase from Plasmodium vivax: complex with NADH

Released:
DOI: 10.2210/pdb2a92/pdb
PDB entry 2a92 coloured by chain, front view.
PDB entry 2a92 coloured by chain, side view.
PDB entry 2a92 coloured by chain, top view.
Source organism: Plasmodium vivax
Primary publication:
Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH.
Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL
Biochemistry 44 16221-8 (2005)
PMID: 16331982

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 34.96 KDa
Source organism: Plasmodium vivax
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4PRK9 (Residues: 2-316; Coverage: 100%)
Gene names: LDH, PVC01_120033300, PVP01_1229700, PVT01_120033900, PVW1_120044200, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 4 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P212121
Unit cell:
a: 81.593Å b: 128.636Å c: 130.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.236
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Potential biomarkers and their applications for rapid and reliable detection of malaria.
Jain et al. (2014)
4 more

5 mentions without citation

Assessing the structural conservation of protein pockets to study functional and allosteric sites: implications for drug discovery.
Panjkovich et al. (2010)
4 more