› 2a94

X-ray diffraction
1.5Å resolution

Structure of Plasmodium falciparum lactate dehydrogenase complexed to APADH.

Released:
DOI: 10.2210/pdb2a94/pdb
PDB entry 2a94 coloured by chain, front view.
PDB entry 2a94 coloured by chain, side view.
PDB entry 2a94 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH.
Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL
Biochemistry 44 16221-8 (2005)
PMID: 16331982

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.85 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27743 (Residues: 2-316; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand AP0 1 x AP0
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I222
Unit cell:
a: 80.183Å b: 86.208Å c: 90.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.176
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Potential biomarkers and their applications for rapid and reliable detection of malaria.
Jain et al. (2014)
3 more

4 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
3 more