› 2a9a

X-ray diffraction
2Å resolution

Crystal structure of recombinant chicken sulfite oxidase with the bound product, sulfate, at the active site

Released:
DOI: 10.2210/pdb2a9a/pdb
PDB entry 2a9a coloured by chain, front view.
PDB entry 2a9a coloured by chain, side view.
PDB entry 2a9a coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural insights into sulfite oxidase deficiency.
Karakas E, Wilson HL, Graf TN, Xiang S, Jaramillo-Busquets S, Rajagopalan KV, Kisker C
J Biol Chem 280 33506-15 (2005)
PMID: 16048997

Function and Biology Details

Reaction catalysed: 
Sulfite + O(2) + H(2)O = sulfate + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfite oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.63 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07850 (Residues: 95-459; Coverage: 80%)
Gene name: SUOX
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand MTE 2 x MTE
2 bound ligands:
Ligand SO4 4 x SO4
Ligand MO 2 x MO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 54.001Å b: 123.17Å c: 55.482Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.162 0.159 0.216
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
7 more

1 mention without citation

QM/MM study of the reaction mechanism of sulfite oxidase.
Caldararu et al. (2018)