› 2a9f

X-ray diffraction
2.5Å resolution

Crystal structure of a putative malic enzyme ((S)-malate:NAD+ oxidoreductase (decarboxylating))

Released:
DOI: 10.2210/pdb2a9f/pdb
PDB entry 2a9f coloured by chain, front view.
PDB entry 2a9f coloured by chain, side view.
PDB entry 2a9f coloured by chain, top view.
Source organism: Streptococcus pyogenes
Entry authors: Seetharaman J, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malic enzyme ((S)-malate:NAD+ oxidoreductase (Decarboxylating)) Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 42.13 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZS1 (Residues: 2-388; Coverage: 100%)
Gene name: SPy_1110
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 68.258Å b: 79.253Å c: 145.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.304
Expression system: Escherichia coli

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