› 2a9o

X-ray diffraction
1.65Å resolution

Crystal structures of an activated YycF homologue, the essential response regulator from S.pneumoniae in complex with BeF3 and the effect of pH on BeF3 binding, possible phosphate in the active site

Released:
DOI: 10.2210/pdb2a9o/pdb
PDB entry 2a9o coloured by chain, front view.
PDB entry 2a9o coloured by chain, side view.
PDB entry 2a9o coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Entry authors: Riboldi-Tunnicliffe A, Isaacs NW, Mitchell TJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein WalR Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.6 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S1K0 (Residues: 1-120; Coverage: 51%)
Gene names: A5N45_11200, AZJ28_06630, AZJ70_09610, AZK02_00045, ERS019261_00996, ERS019316_00372, ERS019486_01573, ERS020302_00624, ERS020893_01557, ERS020941_00881, ERS021825_00456, ERS043904_01196, ERS096071_00083, GM537_00125, GM539_01000, GM543_00370, GM544_00120, GM545_02655, RLD18_04305, SAMEA104154639_01725, SAMEA1462998_01022, SAMEA2627268_02151, SAMEA3171064_01589, SAMEA3353485_01115, SAMEA3353631_00386, SAMEA3354366_01714, SAMEA3381574_00592, SAMEA3389245_00424, SAMEA3390019_02016, SAMEA4038883_00397, micA, rr02, yycF
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2221
Unit cell:
a: 74.563Å b: 93.324Å c: 37.456Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.236
Expression system: Escherichia coli

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