› 2a9z

X-ray diffraction
1.35Å resolution

Crystal structure of T. gondii adenosine kinase complexed with N6-dimethyladenosine and AMP-PCP

Released:
DOI: 10.2210/pdb2a9z/pdb
PDB entry 2a9z coloured by chain, front view.
PDB entry 2a9z coloured by chain, side view.
PDB entry 2a9z coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Substrate analogs induce an intermediate conformational change in Toxoplasma gondii adenosine kinase.
Zhang Y, El Kouni MH, Ealick SE
Acta Crystallogr D Biol Crystallogr 63 126-34 (2007)
PMID: 17242506

Function and Biology Details

Reaction catalysed: 
ATP + adenosine = ADP + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine kinase Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 40.58 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9TVW2 (Residues: 1-363; Coverage: 100%)
Gene name: AK
Sequence domains: pfkB family carbohydrate kinase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand NA 2 x NA
Ligand 26A 2 x 26A
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 60.068Å b: 61.588Å c: 91.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.137 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Adenosine kinase: exploitation for therapeutic gain.
Boison D. (2013)
7 other articles