› 2aa3

X-ray diffraction
2.05Å resolution

Crystal structure of Plasmodium vivax lactate dehydrogenase complex with APADH

Released:
DOI: 10.2210/pdb2aa3/pdb
PDB entry 2aa3 coloured by chain, front view.
PDB entry 2aa3 coloured by chain, side view.
PDB entry 2aa3 coloured by chain, top view.
Source organism: Plasmodium vivax
Primary publication:
Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH.
Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL
Biochemistry 44 16221-8 (2005)
PMID: 16331982

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 34.96 KDa
Source organism: Plasmodium vivax
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4PRK9 (Residues: 2-316; Coverage: 100%)
Gene names: LDH, PVC01_120033300, PVP01_1229700, PVT01_120033900, PVW1_120044200, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand AP0 4 x AP0
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P212121
Unit cell:
a: 81.482Å b: 128.454Å c: 130.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Potential biomarkers and their applications for rapid and reliable detection of malaria.
Jain et al. (2014)
4 more

2 mentions without citation

Use of malaria rapid diagnostic test to identify Plasmodium knowlesi infection.
McCutchan et al. (2008)
1 more