› 2ab5

X-ray diffraction
2.2Å resolution

bI3 LAGLIDADG Maturase

Released:
DOI: 10.2210/pdb2ab5/pdb
PDB entry 2ab5 coloured by chain, front view.
PDB entry 2ab5 coloured by chain, side view.
PDB entry 2ab5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase.
Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM
Nat Struct Mol Biol 12 779-87 (2005)
PMID: 16116439

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b mRNA maturase bI3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 32.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZZW7 (Residues: 256-517; Coverage: 51%)
Gene names: BI3, Q0115
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R, null
Spacegroup: P21
Unit cell:
a: 63.6Å b: 80.2Å c: 66.4Å
α: 90° β: 115.5° γ: 90°
R-values:
R R work R free
0.211 0.208 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Atomic level architecture of group I introns revealed.
Vicens et al. (2006)
2 more