› 2abq

X-ray diffraction
2.1Å resolution

Crystal structure of fructose-1-phosphate kinase from Bacillus halodurans

Released:
DOI: 10.2210/pdb2abq/pdb
PDB entry 2abq coloured by chain, front view.
PDB entry 2abq coloured by chain, side view.
PDB entry 2abq coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry authors: Eswaramoorthy S, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reactions catalysed: 
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tagatose-6-phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 33.19 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KEM5 (Residues: 1-306; Coverage: 100%)
Gene name: fruB
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 42.62Å b: 48.71Å c: 79.05Å
α: 82.38° β: 87.28° γ: 82.14°
R-values:
R R work R free
0.255 0.255 0.299
Expression system: Escherichia coli

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