› 2abw

X-ray diffraction
1.62Å resolution

Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)

Released:
DOI: 10.2210/pdb2abw/pdb
PDB entry 2abw coloured by chain, front view.
PDB entry 2abw coloured by chain, side view.
PDB entry 2abw coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights.
Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Müller S, Macheroux P, Tews I, Kappes B
J Biol Chem 281 3633-41 (2006)
PMID: 16339145

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PDX2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.66 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IIK4 (Residues: 1-219; Coverage: 100%)
Gene names: PDX2, PF11_0169, PF3D7_1116200
Sequence domains: SNO glutamine amidotransferase family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 88.042Å b: 67.917Å c: 70.327Å
α: 90° β: 92.31° γ: 90°
R-values:
R R work R free
0.153 0.152 0.189
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Vitamin B6: a long known compound of surprising complexity.
Mooney et al. (2009)
15 more

6 mentions without citation

Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions.
Barra et al. (2021)
5 more