› 2ac1

X-ray diffraction
2.15Å resolution

Crystal structure of a cell-wall invertase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2ac1/pdb
PDB entry 2ac1 coloured by chain, front view.
PDB entry 2ac1 coloured by chain, side view.
PDB entry 2ac1 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
X-ray diffraction structure of a cell-wall invertase from Arabidopsis thaliana.
Verhaest M, Lammens W, Le Roy K, De Coninck B, De Ranter CJ, Van Laere A, Van den Ende W, Rabijns A
Acta Crystallogr D Biol Crystallogr 62 1555-63 (2006)
PMID: 17139091

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Beta-fructofuranosidase, insoluble isoenzyme CWINV1 Chain: A
Molecule details ›
Chain: A
Length: 541 amino acids
Theoretical weight: 61.47 KDa
Source organism: Arabidopsis thaliana
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q43866 (Residues: 44-584; Coverage: 97%)
Gene names: At3g13790, BFRUCT1, CWINV1, MMM17.26
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 112.93Å b: 162.89Å c: 74.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.243
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
1 more

14 mentions without citation

Integrating bioinformatics tools to handle glycosylation.
Mazola et al. (2011)
13 more