› 2aee

X-ray diffraction
1.95Å resolution

Crystal structure of Orotate phosphoribosyltransferase from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb2aee/pdb
PDB entry 2aee coloured by chain, front view.
PDB entry 2aee coloured by chain, side view.
PDB entry 2aee coloured by chain, top view.
Source organism: Streptococcus pyogenes
Entry authors: Chang C, Li H, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 22.91 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A076 (Residues: 1-209; Coverage: 100%)
Gene names: M5005_Spy0704, SPy_0901, pyrE
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P42212
Unit cell:
a: 118.131Å b: 118.131Å c: 72.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.229
Expression system: Escherichia coli

Quick links