› 2aef

X-ray diffraction
1.7Å resolution

Crystal Structures of the MthK RCK Domain in Ca2+ bound form

Released:
DOI: 10.2210/pdb2aef/pdb
PDB entry 2aef coloured by chain, front view.
PDB entry 2aef coloured by chain, side view.
PDB entry 2aef coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stability.
Dong J, Shi N, Berke I, Chen L, Jiang Y
J Biol Chem 280 41716-24 (2005)
PMID: 16227203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 25.91 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 57.979Å b: 37.908Å c: 96.652Å
α: 90° β: 95.49° γ: 90°
R-values:
R R work R free
0.23 0.228 0.268
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family.
Corratgé-Faillie et al. (2010)
4 more

8 mentions without citation

The RCK2 domain of the human BKCa channel is a calcium sensor.
Yusifov et al. (2008)
7 more