› 2aeo

X-ray diffraction
1.8Å resolution

Crystal structure of cisplatinated bovine Cu,Zn superoxide dismutase

Released:
DOI: 10.2210/pdb2aeo/pdb
PDB entry 2aeo coloured by chain, front view.
PDB entry 2aeo coloured by chain, side view.
PDB entry 2aeo coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural investigation of cisplatin-protein interactions: selective platination of His19 in a cuprozinc superoxide dismutase.
Calderone V, Casini A, Mangani S, Messori L, Orioli PL
Angew Chem Int Ed Engl 45 1267-9 (2006)
PMID: 16416478

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 15.57 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00442 (Residues: 2-152; Coverage: 99%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

3 bound ligands:
Ligand CU 2 x CU
Ligand ZN 2 x ZN
Ligand CPT 2 x CPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCED ULTRA
Spacegroup: P212121
Unit cell:
a: 47.207Å b: 50.92Å c: 146.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.236

Quick links

Citations

5 review citations

Analytical methodologies for metallomics studies of antitumor Pt-containing drugs.
Esteban-Fernández et al. (2010)
4 more

2 mentions without citation

Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin et al. (2015)
1 more