› 2ahr

X-ray diffraction
2.15Å resolution

Crystal Structures of 1-Pyrroline-5-Carboxylate Reductase from Human Pathogen Streptococcus pyogenes

Released:
DOI: 10.2210/pdb2ahr/pdb
PDB entry 2ahr coloured by chain, front view.
PDB entry 2ahr coloured by chain, side view.
PDB entry 2ahr coloured by chain, top view.
Source organism: Streptococcus pyogenes M1 GAS
Primary publication:
Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes.
Nocek B, Chang C, Li H, Lezondra L, Holzle D, Collart F, Joachimiak A
J Mol Biol 354 91-106 (2005)
PMID: 16233902

Function and Biology Details

Reaction catalysed: 
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo decamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrroline-5-carboxylate reductase Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 259 amino acids
Theoretical weight: 27.93 KDa
Source organism: Streptococcus pyogenes M1 GAS
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9A1S9 (Residues: 1-256; Coverage: 100%)
Gene names: SPy_0112, proC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 5 x NAP
2 bound ligands:
Ligand NA 5 x NA
Ligand FMT 5 x FMT
1 modified residue:
Ligand MSE 35 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 171.631Å b: 109.646Å c: 84.033Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.175 0.173 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
5 more

7 mentions without citation

Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes.
Nocek et al. (2005)
6 more