› 2aj4

X-ray diffraction
2.4Å resolution

Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP

Released:
DOI: 10.2210/pdb2aj4/pdb
PDB entry 2aj4 coloured by chain, front view.
PDB entry 2aj4 coloured by chain, side view.
PDB entry 2aj4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer.
Thoden JB, Sellick CA, Timson DJ, Reece RJ, Holden HM
J Biol Chem 280 36905-11 (2005)
PMID: 16115868

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 548 amino acids
Theoretical weight: 60.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P04385 (Residues: 2-528; Coverage: 100%)
Gene names: GAL1, YBR020W, YBR0302
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLA 2 x GLA
Ligand MG 2 x MG
Ligand CL 2 x CL
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 63.3Å b: 85Å c: 112.3Å
α: 90° β: 96.7° γ: 90°
R-values:
R R work R free
0.174 0.172 0.205
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.
Conrad et al. (2014)
3 more

3 mentions without citation

The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy et al. (2012)
2 more