› 2ajq

X-ray diffraction
2.6Å resolution

Structure of replicative DNA polymerase provides insigts into the mechanisms for processivity, frameshifting and editing

Released:
DOI: 10.2210/pdb2ajq/pdb
PDB entry 2ajq coloured by chain, front view.
PDB entry 2ajq coloured by chain, side view.
PDB entry 2ajq coloured by chain, top view.
Source organisms:
Entry authors: Brieba L, Ellenberger T

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115074 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA polymerase Chains: A, F
Molecule details ›
Chains: A, F
Length: 704 amino acids
Theoretical weight: 79.7 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00581 (Residues: 1-704; Coverage: 100%)
Gene name: 5
Sequence domains: DNA polymerase family A
Structure domains:
Thioredoxin 1 Chains: B, I
Molecule details ›
Chains: B, I
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
DNA Primer Chains: P, X
Molecule details ›
Chains: P, X
Length: 22 nucleotides
Theoretical weight: 6.74 KDa
DNA Template Chains: T, Z
Molecule details ›
Chains: T, Z
Length: 26 nucleotides
Theoretical weight: 8.03 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 2DT 2 x 2DT

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2221
Unit cell:
a: 168.312Å b: 169.235Å c: 179.788Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.284
Expression system: Escherichia coli

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