› 2ajt

X-ray diffraction
2.6Å resolution

Crystal structure of L-Arabinose Isomerase from E.coli

Released:
DOI: 10.2210/pdb2ajt/pdb
PDB entry 2ajt coloured by chain, front view.
PDB entry 2ajt coloured by chain, side view.
PDB entry 2ajt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production.
Manjasetty BA, Chance MR
J Mol Biol 360 297-309 (2006)
PMID: 16756997

Function and Biology Details

Reaction catalysed: 
Beta-L-arabinose = L-ribulose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-arabinose isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 500 amino acids
Theoretical weight: 56.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08202 (Residues: 1-500; Coverage: 100%)
Gene names: JW0061, araA, b0062
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 116.539Å b: 116.539Å c: 214.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Tagatose: properties, applications, and biotechnological processes.
Oh DK. (2007)
6 more

6 mentions without citation

PSI-2: structural genomics to cover protein domain family space.
Dessailly et al. (2009)
5 more