› 2akj

X-ray diffraction
2.8Å resolution

Structure of spinach nitrite reductase

Released:
DOI: 10.2210/pdb2akj/pdb
PDB entry 2akj coloured by chain, front view.
PDB entry 2akj coloured by chain, side view.
PDB entry 2akj coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structure of spinach nitrite reductase: implications for multi-electron reactions by the iron-sulfur:siroheme cofactor.
Swamy U, Wang M, Tripathy JN, Kim SK, Hirasawa M, Knaff DB, Allen JP
Biochemistry 44 16054-63 (2005)
PMID: 16331965

Function and Biology Details

Reaction catalysed: 
NH(3) + 2 H(2)O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin--nitrite reductase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 608 amino acids
Theoretical weight: 68.02 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: P05314 (Residues: 33-594; Coverage: 95%)
Gene name: NIR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SRM 1 x SRM
1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 128.97Å b: 128.97Å c: 120.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.3
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl).
Hänsch et al. (2009)
9 more

8 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
7 more