› 2alw

X-ray diffraction
1.86Å resolution

Golgi alpha-mannosidase II complex with Noeuromycin

Released:
DOI: 10.2210/pdb2alw/pdb
PDB entry 2alw coloured by chain, front view.
PDB entry 2alw coloured by chain, side view.
PDB entry 2alw coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The role of the active site Zn in the catalytic mechanism of the GH38 Golgi alpha-mannosidase II: implications from noeuromycin inhibition
Kuntz DA, Liu H, Bols M, Rose DR
Biocatal. Biotransformation 34 55-61 (2006)

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand MNM 1 x MNM
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 68.502Å b: 109.19Å c: 138.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.141 0.181
Expression system: Drosophila melanogaster

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