› 2amv

X-ray diffraction
2.3Å resolution

THE STRUCTURE OF GLYCOGEN PHOSPHORYLASE B WITH AN ALKYL-DIHYDROPYRIDINE-DICARBOXYLIC ACID

Released:
DOI: 10.2210/pdb2amv/pdb
PDB entry 2amv coloured by chain, front view.
PDB entry 2amv coloured by chain, side view.
PDB entry 2amv coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
The structure of glycogen phosphorylase b with an alkyldihydropyridine-dicarboxylic acid compound, a novel and potent inhibitor.
Zographos SE, Oikonomakos NG, Tsitsanou KE, Leonidas DD, Chrysina ED, Skamnaki VT, Bischoff H, Goldmann S, Watson KA, Johnson LN
Structure 5 1413-25 (1997)
PMID: 9384557

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, muscle form Chain: A
Molecule details ›
Chain: A
Length: 842 amino acids
Theoretical weight: 97.29 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P00489 (Residues: 2-843; Coverage: 100%)
Gene name: PYGM
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand BIN 1 x BIN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 127.11Å b: 127.11Å c: 115.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.282

Quick links

Citations

3 review citations

Implications of protein flexibility for drug discovery.
Teague SJ. (2003)
2 more

2 mentions without citation

Chemistry and biology of multicomponent reactions.
Dömling et al. (2012)
1 more