› 2ao5

X-ray diffraction
2.1Å resolution

Crystal structure of an RNA duplex r(GGCGBrUGCGCU)2 with terminal and internal tandem G-U base pairs

Released:
DOI: 10.2210/pdb2ao5/pdb
PDB entry 2ao5 coloured by chain, front view.
PDB entry 2ao5 coloured by chain, side view.
PDB entry 2ao5 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure of an RNA duplex r(GGCGBrUGCGCU)2 with terminal and internal tandem G.U base pairs.
Utsunomiya R, Suto K, Balasundaresan D, Fukamizu A, Kumar PK, Mizuno H
Acta Crystallogr D Biol Crystallogr 62 331-8 (2006)
PMID: 16510980

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196309 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*GP*GP*CP*GP*(5BU)P*GP*CP*GP*CP*U)-3' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 10 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand 5BU 4 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P43
Unit cell:
a: 37.693Å b: 37.693Å c: 96.283Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.207 0.25
Expression system: Not provided

Quick links

Citations

2 citation in other articles

Requirements for gene silencing mediated by U1 snRNA binding to a target sequence.
Abad et al. (2008)
1 more

3 mentions without citation

Coarse-grained model for simulation of RNA three-dimensional structures.
Xia et al. (2010)
2 more