› 2aor

X-ray diffraction
2Å resolution

Crystal structure of MutH-hemimethylated DNA complex

Released:
DOI: 10.2210/pdb2aor/pdb
PDB entry 2aor coloured by chain, front view.
PDB entry 2aor coloured by chain, side view.
PDB entry 2aor coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage.
Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W
Mol Cell 20 155-66 (2005)
PMID: 16209953

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115079 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA mismatch repair protein MutH Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 24.94 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44688 (Residues: 1-223; Coverage: 100%)
Gene names: HI_0403, mutH
Sequence domains: DNA mismatch repair enzyme MutH
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
5'-D(*CP*AP*GP*GP*(6MA)P*TP*CP*CP*AP*AP*GP*CP*TP*TP*GP*GP*AP*TP*CP*CP*TP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.77 KDa
Source organism: Haemophilus influenzae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand 6MA 2 x 6MA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 50.397Å b: 89.045Å c: 75.092Å
α: 90° β: 105.7° γ: 90°
R-values:
R R work R free
0.196 0.196 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Mechanisms and functions of DNA mismatch repair.
Li GM. (2008)
12 more

4 mentions without citation

Chemical trapping of the dynamic MutS-MutL complex formed in DNA mismatch repair in Escherichia coli.
Winkler et al. (2011)
3 more