› 2apf

X-ray diffraction
1.8Å resolution

Crystal Structure of the A52V/S54N/K66E variant of the murine T cell receptor V beta 8.2 domain

Released:
DOI: 10.2210/pdb2apf/pdb
PDB entry 2apf coloured by chain, front view.
PDB entry 2apf coloured by chain, side view.
PDB entry 2apf coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The nitty-gritty of protein interactions.
Schreiber G
Structure 13 1737-8 (2005)
PMID: 16338399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: A2NAI0 (Residues: 1-94; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I212121
Unit cell:
a: 31.608Å b: 74.708Å c: 113.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.197 0.263
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Native top-down mass spectrometry for the structural characterization of human hemoglobin.
Zhang et al. (2015)

2 mentions without citation

Structural basis of affinity maturation and intramolecular cooperativity in a protein-protein interaction.
Cho et al. (2005)
1 more