› 2apq

X-ray diffraction
1.8Å resolution

Crystal Structure of an Active Site Mutant of Bovine Pancreatic Ribonuclease A (H119A-RNase A) with a 10-Glutamine expansion in the C-terminal hinge-loop.

Released:
DOI: 10.2210/pdb2apq/pdb
PDB entry 2apq coloured by chain, front view.
PDB entry 2apq coloured by chain, side view.
PDB entry 2apq coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Amyloid-like fibrils of ribonuclease A with three-dimensional domain-swapped and native-like structure.
Sambashivan S, Liu Y, Sawaya MR, Gingery M, Eisenberg D
Nature 437 266-9 (2005)
PMID: 16148936

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 15.31 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-137, 138-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P3221
Unit cell:
a: 67.228Å b: 67.228Å c: 63.686Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.215
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

The amyloid state of proteins in human diseases.
Eisenberg et al. (2012)
23 more

1 mention without citation

Diffusion, crowding & protein stability in a dynamic molecular model of the bacterial cytoplasm.
McGuffee et al. (2010)