› 2ar0

X-ray diffraction
2.8Å resolution

Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)

Released:
DOI: 10.2210/pdb2ar0/pdb
PDB entry 2ar0 coloured by chain, front view.
PDB entry 2ar0 coloured by chain, side view.
PDB entry 2ar0 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Rajashankar KR, Kniewel R, Lima CD, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type I restriction enzyme EcoKI methylase subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 541 amino acids
Theoretical weight: 61.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08957 (Residues: 2-527; Coverage: 99%)
Gene names: JW4312, b4349, hsdM, hsm
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UNX 10 x UNX
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P41
Unit cell:
a: 105.427Å b: 105.427Å c: 138.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.261
Expression system: Escherichia coli

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