› 2as0

X-ray diffraction
1.8Å resolution

Crystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase

Released:
DOI: 10.2210/pdb2as0/pdb
PDB entry 2as0 coloured by chain, front view.
PDB entry 2as0 coloured by chain, side view.
PDB entry 2as0 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.
Sun W, Xu X, Pavlova M, Edwards AM, Joachimiak A, Savchenko A, Christendat D
Protein Sci 14 3121-8 (2005)
PMID: 16260766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PUA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 45.33 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O59578 (Residues: 1-396; Coverage: 100%)
Gene name: PH1915
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 70.513Å b: 86.161Å c: 76.693Å
α: 90° β: 114.16° γ: 90°
R-values:
R R work R free
0.207 0.207 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The PUA domain - a structural and functional overview.
Pérez-Arellano et al. (2007)
6 other articles

5 mentions without citation

De novo backbone scaffolds for protein design.
MacDonald et al. (2010)
4 more