› 2as5

X-ray diffraction
2.7Å resolution

Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.

Released:
DOI: 10.2210/pdb2as5/pdb
PDB entry 2as5 coloured by chain, front view.
PDB entry 2as5 coloured by chain, side view.
PDB entry 2as5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
FOXP3 controls regulatory T cell function through cooperation with NFAT.
Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A
Cell 126 375-87 (2006)
PMID: 16873067

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115083 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chains: M, N
Molecule details ›
Chains: M, N
Length: 287 amino acids
Theoretical weight: 32.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13469 (Residues: 392-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains:
Structure domains:
Forkhead box protein P2 Chains: F, G
Molecule details ›
Chains: F, G
Length: 93 amino acids
Theoretical weight: 11.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15409 (Residues: 502-594; Coverage: 13%)
Gene names: CAGH44, FOXP2, TNRC10
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(TP*TP*AP*GP*GP*AP*AP*AP*AP*TP*TP*TP*GP*TP*TP*TP*CP*AP*TP*AP*GP*)-3' Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
5'-D(AP*AP*CP*TP*AP*TP*GP*AP*AP*AP*CP*AP*AP*AP*TP*TP*TP*TP*CP*CP*TP*)-3' Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.39 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 65.455Å b: 157.447Å c: 67.666Å
α: 90° β: 118.67° γ: 90°
R-values:
R R work R free
0.238 0.238 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

266 review citations

Regulatory T cells and immune tolerance.
Sakaguchi et al. (2008)
265 more

8 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
7 more