› 2ash

X-ray diffraction
1.9Å resolution

Crystal structure of Queuine tRNA-ribosyltransferase (EC 2.4.2.29) (tRNA-guanine (tm1561) from THERMOTOGA MARITIMA at 1.90 A resolution

Released:
DOI: 10.2210/pdb2ash/pdb
PDB entry 2ash coloured by chain, front view.
PDB entry 2ash coloured by chain, side view.
PDB entry 2ash coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 42.95 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1P7 (Residues: 1-369; Coverage: 100%)
Gene names: TM_1561, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 5 x CL
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1, ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 169.467Å b: 99.422Å c: 124.163Å
α: 90° β: 123.8° γ: 90°
R-values:
R R work R free
0.195 0.193 0.229
Expression system: Escherichia coli

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