› 2ast

X-ray diffraction
2.3Å resolution

Crystal structure of Skp1-Skp2-Cks1 in complex with a p27 peptide

Released:
DOI: 10.2210/pdb2ast/pdb
PDB entry 2ast coloured by chain, front view.
PDB entry 2ast coloured by chain, side view.
PDB entry 2ast coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase.
Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP
Mol Cell 20 9-19 (2005)
PMID: 16209941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
PDBe Complex ID:
PDB-CPX-155477 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 2-160; Coverage: 98%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
S-phase kinase-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13309 (Residues: 89-424; Coverage: 79%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Structure domains: Ribonuclease Inhibitor
Cyclin-dependent kinases regulatory subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 69 amino acids
Theoretical weight: 8.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61024 (Residues: 5-73; Coverage: 87%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cyclin-dependent kinase, regulatory subunit
Cyclin-dependent kinase inhibitor 1B Chain: D
Molecule details ›
Chain: D
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46527 (Residues: 181-190; Coverage: 5%)
Gene names: CDKN1B, KIP1, p27

Ligands and Environments

1 bound ligand:
Ligand BEN 2 x BEN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 148.7Å b: 148.7Å c: 98.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

43 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
42 more

31 mentions without citation

Structural insights into the functional diversity of the CDK-cyclin family.
Wood et al. (2018)
30 more