› 2at8

X-ray diffraction
1Å resolution

0.96 A Crystal Structure Of Nitrophorin 4 From Rhodnius Prolixus Containing Fe(III) 2,4 Dimethyl Deuteroporphyrin IX Complexed With Nitric Oxide at pH 5.6

Released:
DOI: 10.2210/pdb2at8/pdb
PDB entry 2at8 coloured by chain, front view.
PDB entry 2at8 coloured by chain, side view.
PDB entry 2at8 coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Amoia AM, Montfort WR

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: X
Molecule details ›
Chain: X
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand FDD 1 x FDD
Ligand NO 1 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 70.211Å b: 42.582Å c: 52.935Å
α: 90° β: 94.61° γ: 90°
R-values:
R R work R free
0.166 0.165 0.195
Expression system: Escherichia coli BL21(DE3)

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