› 2atk

X-ray diffraction
2.5Å resolution

Structure of a mutant KcsA K+ channel

Released:
DOI: 10.2210/pdb2atk/pdb
PDB entry 2atk coloured by chain, front view.
PDB entry 2atk coloured by chain, side view.
PDB entry 2atk coloured by chain, top view.
Source organisms:
Primary publication:
Molecular determinants of gating at the potassium-channel selectivity filter.
Cordero-Morales JF, Cuello LG, Zhao Y, Jogini V, Cortes DM, Roux B, Perozo E
Nat Struct Mol Biol 13 311-8 (2006)
PMID: 16532009

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209443 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.15 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand K 7 x K
Ligand F09 1 x F09
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 155.16Å b: 155.16Å c: 75.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.261
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

31 review citations

Bringing dynamic molecular machines into focus by methyl-TROSY NMR.
Rosenzweig et al. (2014)
30 more

12 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
11 more