Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Type II restriction enzyme HincII (preferred)
PDBe Complex ID:
PDB-CPX-148157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme HincII
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
Sequence domains: Restriction endonuclease HincII
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
- Canonical:
P17743 (Residues: 2-258; Coverage: 100%)
Sequence domains: Restriction endonuclease HincII
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 14-BM-D
Spacegroup:
P43212
Expression system: Escherichia coli
