› 2awa

X-ray diffraction
2.5Å resolution

Crystal structure of DNA polymerase III, beta chain (EC 2.7.7.7) (np_344555.1) from STREPTOCOCCUS PNEUMONIAE TIGR4 at 2.50 A resolution

Released:
DOI: 10.2210/pdb2awa/pdb
PDB entry 2awa coloured by chain, front view.
PDB entry 2awa coloured by chain, side view.
PDB entry 2awa coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 390 amino acids
Theoretical weight: 43.76 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: O06672 (Residues: 1-378; Coverage: 100%)
Gene names: SP_0002, dnaN
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 94.06Å b: 70.72Å c: 135.44Å
α: 90° β: 91.04° γ: 90°
R-values:
R R work R free
0.195 0.192 0.25
Expression system: Escherichia coli

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